Manipulation of carbon media, temperature and hydraulic efficiency to increase nitrate removal rate in denitrification beds

The accumulation of reactive nitrogen (Nr) in terrestrial and aquatic environments is a global environmental issue that causes or contributes to climate change, stratospheric ozone depletion, and deterioration of coastal and terrestrial waters. Point source discharges of Nr from municipal and septic treatment systems, agricultural tile drainage, and industrial discharges contribute to these issues. Practical, low-cost methods are needed to reduce the Nr load into the environment from small-volume point source discharges. Denitrification beds are one such method. Improving the nitrate removal rate of denitrification beds will lead to reduced bed volumes, lower construction costs that likely facilitate greater uptake of the technology and reduced accumulation of Nr in the environment. The main objective of this thesis was to test a number of approaches that might increase the rate of nitrate removal rate in a denitrification bed under non-nitrate limiting conditions, including: manipulation of carbon source, temperature and hydraulic flow. To date, operational denitrification beds have used wood media as the carbon source which sustains nitrate removal rates of between 2–10 g N m-3 of media d-1 and relatively high permeability. While previous laboratory experiments have investigated the potential of alternative carbon sources, these studies were typically of short duration and small scale and did not necessarily provide reliable information for denitrification bed design purposes. To address this issue, nitrate removal, hydraulic and nutrient leaching characteristics of nine different carbon substrates were compared in 0.2 m3 barrels, at 14oC and 23.5oC over a 23 month period. The relationship between hydraulic efficiency and nitrate removal of the different media was also investigated. Findings from the barrel trial were field tested in pilot scale (2.9 m3) denitrification beds receiving municipal effluent dosed with KNO3, over a 15 month period. The pilot scale trial tested whether nitrate removal could be improved by using an alternative carbon media (maize cobs) and increasing bed temperature through passive solar heating. The influence of bed flow regime (horizontal-point, horizontal-diffuse, downflow and upflow) on hydraulic efficiency and nitrate removal was also investigated. This thesis demonstrated that more labile carbon sources, such as maize cobs, had significantly higher nitrate removal rates (15.0 to 21.8 g N m-3 d-1) than wood media (3.0 to 4.9 g N m-3 d-1) over the duration of the barrel trial. Nitrate removal rates increased with increasing temperature with mean Q10 of 1.6 for all media. The hydraulic efficiency of fragmented wood media decreased with increasing grain-size. However, nitrate removal rate was not dependent on hydraulic efficiency of the media, which was attributed to the significant secondary porosity of the media allowing denitrification to occur both on the surface and within the media particle. In the pilot scale trial, bed temperature increased by 3.4oC due to passive solar heating, but did not cause a measureable increase in nitrate removal rate due to variability in removal rates and possibly low temperature responsiveness of maize cobs for removing nitrate. Flow regime affected the hydraulic efficiency of denitrification beds and nitrate removal rates were lower in flow regimes with poor hydraulic efficiency. This was attributed to short-circuit flow reducing the bed volume that contributed to nitrate removal. The results indicate that a four-fold reduction in denitrification bed size could potentially be achieved by using maize cobs as the carbon substrate, as opposed to wood fragments, and increasing bed temperature by incorporating passive solar heating techniques. The findings of this thesis indicate that future research on improving the nitrate removal rate of denitrification beds under non-nitrate limiting conditions should focus on carbon substrates, increasing bed temperature, and hydraulic design of beds rather than on hydraulic efficiency of media. For example, research on coupling improved solar heating design with an appropriate inlet/outlet structure and location

An early giant planet instability recorded in asteroidal meteorites

Giant planet migration appears widespread among planetary systems in our Galaxy. However, the timescales of this process, which reflect the underlying dynamical mechanisms, are not well constrained, even within the solar system. Since planetary migration scatters smaller bodies onto intersecting orbits, it would have resulted in an epoch of enhanced bombardment in the solar system's asteroid belt. To accurately and precisely quantify the timescales of migration, we interrogate thermochronologic data from asteroidal meteorites, which record the thermal imprint of energetic collisions. We present a database of 40K-40Ar system ages from chondrite meteorites and evaluate it with an asteroid-scale thermal code coupled to a Markov chain Monte Carlo inversion. Simulations require bombardment in order to reproduce the observed age distribution and identify a bombardment event beginning ~11 million years after the Sun formed. Our results associate a giant planet instability in our solar system with the dissipation of the gaseous protoplanetary disk.Comment: 24 pages, 4 figures, 2 tables, 10 extended data items (8 figures, 2 tables). Under review at Nature Astronom

Investigation of salicylate hepatic responses in comparison with chemical analogues of the drug

AbstractAnti-hyperglycaemic effects of the hydroxybenzoic acid salicylate might stem from effects of the drug on mitochondrial uncoupling, activation of AMP-activated protein kinase, and inhibition of NF-κB signalling. Here, we have gauged the contribution of these effects to control of hepatocyte glucose production, comparing salicylate with inactive hydroxybenzoic acid analogues of the drug. In rat H4IIE hepatoma cells, salicylate was the only drug tested that activated AMPK. Salicylate also reduced mTOR signalling, but this property was observed widely among the analogues. In a sub-panel of analogues, salicylate alone reduced promoter activity of the key gluconeogenic enzyme glucose 6-phosphatase and suppressed basal glucose production in mouse primary hepatocytes. Both salicylate and 2,6 dihydroxybenzoic acid suppressed TNFα-induced IκB degradation, and in genetic knockout experiments, we found that the effect of salicylate on IκB degradation was AMPK-independent. Previous data also identified AMPK-independent regulation of glucose but we found that direct inhibition of neither NF-κB nor mTOR signalling suppressed glucose production, suggesting that other factors besides these cell signalling pathways may need to be considered to account for this response to salicylate. We found, for example, that H4IIE cells were exquisitely sensitive to uncoupling with modest doses of salicylate, which occurred on a similar time course to another anti-hyperglycaemic uncoupling agent 2,4-dinitrophenol, while there was no discernible effect at all of two salicylate analogues which are not anti-hyperglycaemic. This finding supports much earlier literature suggesting that salicylates exert anti-hyperglycaemic effects at least in part through uncoupling

Study Protocol for RESORP – Resolution of Organ Injury in Acute Pancreatitis – an observational prospective cohort study

Introduction Survivors of acute pancreatitis (AP) have shorter overall survival and increased incidence of new-onset cardiovascular, respiratory, liver and renal disease, diabetes mellitus and cancer compared with the general population, but the mechanisms that explain this are yet to be elucidated. Our aim is to characterise the precise nature and extent of organ dysfunction following an episode of AP.Methods and analysis This is an observational prospective cohort study in a single centre comprising a University hospital with an acute and emergency receiving unit and clinical research facility. Participants will be adult patient admitted with AP. Participants will undergo assessment at recruitment, 3 months and 3 years. At each time point, multiple biochemical and/or physiological assessments to measure cardiovascular, respiratory, liver, renal and cognitive function, diabetes mellitus and quality of life. Recruitment was from 30 November 2017 to 31 May 2020; last follow-up measurements is due on 31 May 2023. The primary outcome measure is the incidence of new-onset type 3c diabetes mellitus during follow-up. Secondary outcome measures include: quality of life analyses (SF-36, Gastrointestinal Quality of Life Index); montreal cognitive assessment; organ system physiological performance; multiomics predictors of AP severity, detection of premature cellular senescence. In a nested cohort within the main cohort, individuals may also consent to multiparameter MRI scan, echocardiography, pulmonary function testing, cardiopulmonary exercise testing and pulse-wave analysis.Ethics and dissemination This study has received the following approvals: UK IRAS Number 178615; South-east Scotland Research Ethics Committee number 16/SS/0065. Results will be made available to AP survivors, caregivers, funders and other researchers. Publications will be open-access.Trial registration numbers ClinicalTrials.gov Registry (NCT03342716) and ISRCTN50581876; Pre-results

The Canberra Commission: Paths Followed, Paths Ahead

Despite its inauspicious start and virtual abandonment by the new Coalition government in Australia, the Canberra Commission on the Elimination of Nuclear Weapons continued to attract international attention in arms control and disarmament circles

The Quaternary geology of the North Sea basin

The North Sea is a shallow (∼50–400 m deep), ∼500-km-wide marine embayment that separates the UK from Scandinavia and northern Europe (Fig. 1). This epicontinental shelf area has had a long and complex geological history with its present-day structural configuration largely being the result of rifting during the Jurassic–Early Cretaceous, followed by thermal subsidence (Glennie and Underhill, 1998; Zanella and Coward, 2003). Since the middle Cenozoic, the Central Graben region of the North Sea basin has accumulated up to 3000 m of Oligocene to Holocene sediments, which locally includes more than 800 m of Quaternary sediments (Caston, 1977, 1979; Gatliff et al., 1994). Although a detailed understanding of the depositional history recorded by this sedimentary succession is yet to be fully established, these sediments preserve evidence for the advance and retreat of several ice sheets into the North Sea from the adjacent landmasses at different times during the Quaternary. These ice masses not only resulted in periodic erosion, but also made a significant depositional contribution to the infill of the basin

A review of elliptical and disc galaxy structure, and modern scaling laws

A century ago, in 1911 and 1913, Plummer and then Reynolds introduced their models to describe the radial distribution of stars in `nebulae'. This article reviews the progress since then, providing both an historical perspective and a contemporary review of the stellar structure of bulges, discs and elliptical galaxies. The quantification of galaxy nuclei, such as central mass deficits and excess nuclear light, plus the structure of dark matter halos and cD galaxy envelopes, are discussed. Issues pertaining to spiral galaxies including dust, bulge-to-disc ratios, bulgeless galaxies, bars and the identification of pseudobulges are also reviewed. An array of modern scaling relations involving sizes, luminosities, surface brightnesses and stellar concentrations are presented, many of which are shown to be curved. These 'redshift zero' relations not only quantify the behavior and nature of galaxies in the Universe today, but are the modern benchmark for evolutionary studies of galaxies, whether based on observations, N-body-simulations or semi-analytical modelling. For example, it is shown that some of the recently discovered compact elliptical galaxies at 1.5 < z < 2.5 may be the bulges of modern disc galaxies.Comment: Condensed version (due to Contract) of an invited review article to appear in "Planets, Stars and Stellar Systems"(www.springer.com/astronomy/book/978-90-481-8818-5). 500+ references incl. many somewhat forgotten, pioneer papers. Original submission to Springer: 07-June-201

Metformin selectively targets redox control of complex I energy transduction

Many guanide-containing drugs are antihyperglycaemic but most exhibit toxicity, to the extent that only the biguanide metformin has enjoyed sustained clinical use. Here, we have isolated unique mitochondrial redox control properties of metformin that are likely to account for this difference. In primary hepatocytes and H4IIE hepatoma cells we found that antihyperglycaemic diguanides DG5-DG10 and the biguanide phenformin were up to 1000-fold more potent than metformin on cell signalling responses, gluconeogenic promoter expression and hepatocyte glucose production. Each drug inhibited cellular oxygen consumption similarly but there were marked differences in other respects. All diguanides and phenformin but not metformin inhibited NADH oxidation in submitochondrial particles, indicative of complex I inhibition, which also corresponded closely with dehydrogenase activity in living cells measured by WST-1. Consistent with these findings, in isolated mitochondria, DG8 but not metformin caused the NADH/NAD+ couple to become more reduced over time and mitochondrial deterioration ensued, suggesting direct inhibition of complex I and mitochondrial toxicity of DG8. In contrast, metformin exerted a selective oxidation of the mitochondrial NADH/NAD+ couple, without triggering mitochondrial deterioration. Together, our results suggest that metformin suppresses energy transduction by selectively inducing a state in complex I where redox and proton transfer domains are no longer efficiently coupled